Antinuclear Antibody (ANA)

Department of Immunology

Notes

• This test is used as an initial screen to assess the need for more specific tests (ENA and anti-DNA antibodies) which are automatically carried out on ANA positive samples.
• ANA in the absence of ENA or anti-DNA is usually of no specific diagnostic value, though it can suggest evolving connective tissue disease which will later be characterised by more specific autoantibodies.
• ANA can be positive in patients with Systemic Lupus erythematosus, Sjogren’s, Scleroderma and other connective tissue disorders.
• Cytoplasmic staining patterns can also be observed in patients with Primary Biliary Cirrhosis and in such cases an autoimmune profile will be added.

Sample Requirements

Clotted blood. (No patient preparation or special handling required).

Adult

5ml Gold-top SST tube or 3.5ml Rust-top gel tube (Trust users only)

Paediatric

1ml Serum sample

Turnaround Times

5 days

Time Limit for Add-ons

3 days

Minimum Retest Interval

3 months

Reference Range

Normal = Negative