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Dispatch of samples to other laboratories

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Red cell Antibodies, RBC typing, crossmatching

Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
Preferably 2 x 6mL EDTA Red Cell Immunology, NBS Filton, 500 North Bristol Park, Northway, Filton, Bristol, BS34 7QH RCI Form 1A (Blue on White form) 5 working days

HLA Typing (Diagnostic Narcolepsy, Irirtis, uveitis, Behcets Syndrome etc. (HLA B27)

Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
6mL EDTA or citrate sample HLA Typing Laboratory, Southmead Hospital, Westbury on Trym, Bristol, BS10 5NB North Bristol NHS Trust Request for HLA investigation Form. (Sent from Immunology at GRH) 14 working days DELETE

HLA-B5701 (abacavir Hypersensitivity)

Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
6mL EDTA or citrate sample Department of Immunlolgy, Southmead Hospital, Westbury on Trym, Bristol, BS10 5NB Request for HLA Type: Abacavir Hypersensitivity 14 working days DELETE

HPA antibody screen

Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
6mL Clot Histocompatibility & Immunogenetics NBS Filton, 500 North Bristol Park Northway, Filton, Bristol BS34 7QH Histocompatibility& Immunogenetics - Diagnostic laboratory Form 3a (purple) 7 working days

HPA typing (Platelet Ag)

Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
6mL EDTA Histocompatibility & Immunogenetics NBS Filton, 500 North Bristol Park Northway, Filton, Bristol BS34 7QH Histocompatibility& Immunogenetics - Diagnostic laboratory Form 3a (purple) 7 working days

HNA Antibody screen

Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
6mL clot Histocompatibility & Immunogenetics NBS Filton, 500 North Bristol Park Northway, Filton, Bristol BS34 7QH Histocompatibility& Immunogenetics - Diagnostic laboratory Form 3a (purple) 7 working days

HNA Typing (Neutrophil Ag)

Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
6mL EDTA Histocompatibility & Immunogenetics NBS Filton, 500 North Bristol Park Northway, Filton, Bristol BS34 7QH Histocompatibility& Immunogenetics - Diagnostic laboratory Form 3a (purple) 7 working days

HLA antibody screen (renal Transplant Pt’s)

Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
6mL Clot Histocompatibility & Immunogenetics NBS Filton, 500 North Bristol Park Northway, Filton, Bristol BS34 7QH Histocompatibility& Immunogenetics - Diagnostic laboratory Form 3a (purple) 7 working days

TRALI Investigation

Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
Pre transfusion Sample 2x6mL clot, 2x6mL EDTA Histocompatibility & Immunogenetics NBS Filton, 500 North Bristol Park Northway, Filton, Bristol BS34 7QH Histocompatibility& Immunogenetics - Diagnostic laboratory Form 3a (purple) 7 working days

Post Transfusion Purpura (PTP)

Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
6mL EDTA and 6 mL Clot Histocompatibility & Immunogenetics NBS Filton, 500 North Bristol Park Northway, Filton, Bristol BS34 7QH Histocompatibility& Immunogenetics - Diagnostic laboratory Form 3a (purple) 7 working days

Severe febrile non haemolytic transfusion reaction screening

Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
2x6mL EDTA and 2 x 6mL clot Histocompatibility & Immunogenetics NBS Filton, 500 North Bristol Park Northway, Filton, Bristol BS34 7QH Histocompatibility& Immunogenetics - Diagnostic laboratory Form 3a (purple) 7 working days

Transfusion-associated GvH disease (STR testing)

Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
6mL clot + 6mL EDTA Histocompatibility & Immunogenetics NBS Filton, 500 North Bristol Park Northway, Filton, Bristol BS34 7QH Histocompatibility& Immunogenetics - Diagnostic laboratory Form 3a (purple) 7 working days

Platelet refractorines

Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
6mL Clot & 6mL EDTA Histocompatibility & Immunogenetics NBS Filton, 500 North Bristol Park Northway, Filton, Bristol BS34 7QH Histocompatibility& Immunogenetics - Diagnostic laboratory Form 3a (purple) 7 working days

Therapeutic HLA Typing e.g. BMT patients and potential Donors

Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
6mL EDTA Histocompatibility & Immunogenetics NBS Filton,500 North Bristol Park Northway, Filton, Bristol, BS34 7QH Histocompatibility & Immunogenetics Haematopoietic Stem Cell Transplantation (HSCT Patients and Donors) Form 3c (blue) 10 working days

Chimerism analysis

Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
6mL EDTA Histocompatibility & Immunogenetics NBS Filton,500 North Bristol Park Northway, Filton, Bristol, BS34 7QH Histocompatibility & Immunogenetics Haematopoietic Stem Cell Transplantation (HSCT Patients and Donors) Form 3c (blue) 10 working days

Autoimmune ITP

Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
3 x 6mL EDTA & 6mL clot Histocompatibility & Immunogenetics, Platelet Immunology Laboratory, NBS Filton 500 North Bristol Park, Northway, Filton, Bristol, BS34 7QH Histocompatibility & Immunogenetics Platelet Immunology form 3D (orange) 5 working days

Fetal Neonatal Alloimmune thrombocytopenia (FNAITP)

Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
Maternal: 1 x 6 mL EDTA & 6 mL clot Paternal: 1x 6mL EDTA Neonate: 1mL EDTA Histocompatibility & Immunogenetics, Platelet Immunology Laboratory, NBS Filton 500 North Bristol Park, Northway, Filton, Bristol, BS34 7QH Histocompatibility & Immunogenetics Platelet Immunology form 3D (orange) 5 working days

Heparin Induced Thrombocytopenia (HIT)

Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
6ml clot Histocompatibility & Immunogenetics, Platelet Immunology Laboratory, NBS Filton 500 North Bristol Park, Northway, Filton, Bristol, BS34 7QH Histocompatibility & Immunogenetics Platelet Immunology form 3D (orange) Results from 3hrs from sample receipt by fax, 5 working days for written report
Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
6mL EDTA & 6mL clot & sample of implicated drug Histocompatibility & Immunogenetics, Platelet Immunology Laboratory, NBS Filton 500 North Bristol Park, Northway, Filton, Bristol, BS34 7QH Histocompatibility & Immunogenetics Platelet Immunology form 3D (orange) Up to 21 working days

Autoimmune Neutropenia

Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
6mL clot Histocompatibility & Immunogenetics Platelet & Granulocyte Immunology Laboratory NBS Filton, 500 North Bristol Park, Northway, Filton, Bristol, BS34 7QH Histocompatibility & Immunogenetics granulocyte Immunology form 3E (green) Up to 21 working days
Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
6mL clot & sample of implicated drug Histocompatibility & Immunogenetics Platelet & Granulocyte Immunology Laboratory NBS Filton, 500 North Bristol Park, Northway, Filton, Bristol, BS34 7QH Histocompatibility & Immunogenetics granulocyte Immunology form 3E (green) Up to 21 working days

Neonatal allo-immune Neutropenia

Samples Required Testing Laboratory Request Form Required Expected Turn- around time.
Maternal: 1 x 6 mL EDTA & 6 mL clot Paternal: 1x 6mL EDTA Neonate: 1mL EDTA Histocompatibility & Immunogenetics Platelet & Granulocyte Immunology Laboratory NBS Filton, 500 North Bristol Park, Northway, Filton, Bristol, BS34 7QH Histocompatibility & Immunogenetics granulocyte Immunology form 3E (green) Up to 21 working days
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