Meningo-encephalitis viral PCR screen on CSF

Microbiology

Notes

• The information given here is intended for use by healthcare professionals. Please see Lab Tests Online-UK for more general advice, links and background.
• Used for patients with suspected viral meningo-encephalitis
• The test is usually performed on a lymphocytic CSF
• This screen comprises:
  • Enterovirus PCR
  • Herpes simplex PCR
  • Varicella zoster PCR
• Other viral PCR tests may be available after discussion with the Consultant Microbiologist, e.g. HHV6 in children, CMV in immuno-suppressed patients etc.
• The information given here is intended for use by healthcare professionals. Please see Lab Tests Online-UK for more general advice, links and background.

Sample requirements

This will be performed on selected CSF samples sent for microscopy and culture.

See Cerebrospinal fluid (CSF)

A minimum CSF volume of 200 µl per assay is required for reliable testing. Smaller volumes down to a minimum of 50 µl can be tested, but assay sensitivity may be reduced.

Required information

• Relevant clinical details
• Date of onset
• Length of history
• Prior administration of antibiotics
• Contact with other suspected cases
• Vaccination history

Storage/transport

Refrigerate immediately after collection

Transport to the laboratory without delay

See CSF transport flowchart

Turnaround time

Sent to a Regional Reference Centre

Up to 7 days