Mycoplasma genitalium PCR
Microbiology
Notes
- The information given here is intended for use by healthcare professionals. Please see Lab Tests Online-UK for more general advice, links and background.
 - Testing is typically arranged via Gloucestershire Sexual Health Services (GU Medicine).
 - The test is only validated for male urine samples and vulvo-vaginal swabs. These can be selftaken.
 - Urine samples from female patients are relatively insensitive and are not recommended.
 - This test is for Mycoplasma genitalium in genital tract samples. There is no nationally available test for Ureaplasma spp or Mycoplasma hominis in genital tract samples.
 - For Mycoplasma pneumoniae as a cause of respiratory infection see Mycoplasma pneumoniae
 
Sample requirements


Urine
- Collect 10–30 mL of a first catch urine sample (a minimum of 5 mL urine is required) in cisgender men
 - Prior to sampling, the patient should not have urinated for at least an hour.
 - Boric acid containers are NOT suitable.
 
Swab
- Vulvo-vaginal Swab
 - A clinician or self-taken vaginal swab in cisgender women.
 

Required information
- Please provide relevant clinical details of urethritis, cervicitis, PID, dates of contact with other cases. Antibiotic exposure.
 - Consider referral to Sexual Health Services and testing for HIV.
 
Storage/transport
- Store urine at 2–30°C for up to 24 hours.
 - Store transferred urines and swabs in their Aptima transport medium at 2–30°C. These remain viable for up to 30 days.
 - Transport as soon as possible at ambient temperature.
 
Turnaround time
Typically tested twice a week so up to 4 days.