Skin Antibodies (Pemphigus / Pemphigoid)

Department of Immunology

Notes

• Useful in the diagnosis of autoimmune bullous skin diseases (pemphigus vulgaris and bullous pemphigoid).
• Indirect Immunofluorescence testing (first line test) performed at the Immunology Department, Southmead Hospital.
• Secondary ELISA testing for BP180, BP230, Desmoglein and Anti-Collagen VII is referred to St Johns Institute of Dermatology. This may be reflex tested in challenging cases or can be requested if clinically indicated.

Sample Requirements

Clotted blood. (No patient preparation or special handling required)

Adult

5ml Gold-top SST tube or 3.5ml Rust-top gel tube (Trust users only)

Paediatric

1ml Serum Sample

Turnaround Times

21 days

Time Limit for Add-ons

3 days

Reference Range

IIF - Normal = Negative

ELISA’s - See individual reports for ranges.