Antinuclear Antibody (ANA)

Department of Immunology

Notes

• This test is used as an initial screen to assess the need for more specific tests (ENA and anti-DNA antibodies) which are automatically carried out on ANA positive samples.
• ANA in the absence of ENA or anti-DNA is usually of no specific diagnostic value, though it can suggest evolving connective tissue disease which will later be characterised by more specific autoantibodies.
• ANA can be positive in patients with Systemic Lupus erythematosus, Sjogren’s, Scleroderma and other connective tissue disorders.
• Cytoplasmic staining patterns can also be observed in patients with Primary Biliary Cirrhosis and in such cases an autoimmune profile will be added.

Sample Requirements

Adult

5ml Gold-top SST tube or 3.5ml Rust-top gel tube (Trust users only)

Paediatric

1ml Serum sample

Turnaround Times

1 week

Time Limit for Add-ons

4 days (whilst stored at 2°C - 8 °C)